Macrophages-Hepatocyte Coculture Model Development Service

Unlocking Liver Insights: Together in Culture

Creative Biolabs offers comprehensive In Vitro Coculture Model Development Services, providing tailored solutions to meet our clients' research needs. The development of macrophages-hepatocyte coculture technology provides essential tools for studying cellular interactions within the liver, enabling a deeper understanding of the pathogenesis of liver diseases and the mechanisms of drug action.

Our Technologies

Macrophages-hepatocyte coculture technology encompasses various methods aimed at co-culturing macrophages and hepatocytes within the same in vitro environment to simulate their interactions as observed in the liver. The following are some common techniques. The choice of these techniques depends on the specific research objectives, available resources, and experimental conditions.

Conventional Dual-cell Co-culture Technique: Direct co-culture of macrophages and hepatocytes in the same culture dish. This method is simple but may not fully replicate the complex cellular microenvironment of the liver.
Three-dimensional Cell Co-culture Technique: Co-culturing macrophages and hepatocytes in a three-dimensional manner using scaffolds or matrix materials. This approach closely mimics the structure of the liver and provides a more realistic cellular interaction environment.
Microfluidic Chip Technology: Co-culturing macrophages and hepatocytes on microfluidic chips, allowing precise control of cell-cell interactions at a microscale level and providing higher experimental reproducibility.
Organ-on-a-chip Technology: Co-culturing macrophages and hepatocytes in organ-on-a-chip models such as liver organoids or liver microenvironment models. This method better replicates the structure and function of the liver in vitro.
Transgenic Technology: Modifying the expression of specific genes in macrophages or hepatocytes using transgenic techniques to simulate particular physiological or pathological states, such as inflammatory responses or liver diseases.

Advanced cell coculture methods including optimization of culture media and cell passaging ensure robust growth and stability of both macrophages and hepatocytes. After coculture, isolation, tissue engineering, and functional analysis will also be included.

Cell Isolation and Purification	Tissue Engineering	Functional Analysis
Employing techniques for cell isolation and purification to obtain high-purity hepatocytes and macrophages from tissue samples or cell lines, ensuring accuracy and reproducibility of the culture model.	Integration of tissue engineering techniques to construct three-dimensional cell culture models that better mimic the microenvironment and cellular interactions in the liver.	Utilization of functional analysis techniques such as metabolic activity assays and cytotoxicity assessments to evaluate the stability and biological activity of the culture model.

Applications of Macrophage-Hepatocyte Coculture Model

Drug Screening and Development: Assessment of drug metabolism, toxicity, and efficacy in the liver, accelerating the drug development process.
Disease Modeling and Mechanistic Studies: Modeling the progression of diseases such as hepatitis, liver fibrosis, and liver cancer to investigate related pathophysiological mechanisms.
Immunomodulation Research: Investigating the role of macrophages in liver immune regulation to gain insights into the pathogenesis of immune-related diseases.
Personalized Medicine: Establishment of personalized in vitro models using patient-derived hepatocytes for the development of individualized treatment strategies.

Advantages

Biological Relevance: Mimicking the interactions between macrophages and hepatocytes in the liver, the model possesses high biological relevance, enabling more accurate prediction of drug metabolism, toxicity, and immune responses.
Highly Controllable: Adjustable culture conditions allow simulation of various pathophysiological states such as liver fibrosis, hepatocellular carcinoma, etc., providing a more realistic and reliable platform for disease-related research.
High-Throughput Screening: Suitable for drug screening and toxicity assessment, enabling high-throughput experimental design and data acquisition, thereby expediting the drug development process.
Personalized Medicine: Integration of patient-derived hepatocytes supports personalized medicine research, laying the groundwork for precision medicine approaches.

Publications Sharing

Technology: Colony forming unit (CFU) assays

Journal: BMC Microbiology

IF: 4.2

Research Findings: Utilizing a human macrophage-hepatocyte co-culture model, the researchers investigated the course of infection of three strains of Francisella tularensis and analyzed the immune response triggered upon infection. The study demonstrated that this co-culture model is a suitable tool for in vitro studies on the immune response to liver cell infection.

Fig.1 Presence and replication of intracellular viable bacteria in the macrophage-hepatocyte co-cultures. (Knut, et al., 2016) Fig.1 Quantification of colony forming units of macrophages-hepatocyte coculture.¹

Unlock the potential of your research with macrophage-hepatocyte coculture model development at Creative Biolabs. Our expertise in this field ensures accurate modeling of complex cellular interactions, paving the way for deeper insights into liver biology and disease mechanisms. Contact us to discuss how we can tailor our macrophage-hepatocyte coculture model development services to meet your specific research needs.

Reference

Rennert, Knut, et al. "A human macrophage–hepatocyte co-culture model for comparative studies of infection and replication of Francisella tularensis LVS strain and subspecies holarctica and mediasiatica." BMC microbiology 16 (2016): 1-10.