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Rat Macrophages, Liver, 1-10 x 10^6 (MTS-0922-JF92)

Q: Can these cells reliably mimic Kupffer cell behavior in drug-induced liver injury studies?

Yes. These liver-derived macrophages act as a robust in vitro surrogate for Kupffer cells. They sense DAMPs and PAMPs, respond strongly to endotoxin and hepatotoxic compounds, and release key mediators such as TNF-α, IL-1β, and IL-6.

Datasheet

Overview

Description

This product is isolated from the liver of pathogen-free laboratory rat. 1-10 x 10^6 cells are delivered frozen. This product is negative in the tests of mycoplasma, bacteria, fungi and yeast. The expression of CD11b is positive by flow cytometry. It is guaranteed to further culture in the conditions provided by Creative Biolabs. Repeated freezing and thawing of cells is not recommended.

Applications

Numerous types of experimental manipulations, including morphological, gene expression, and physiological studies.

Species

Rat

Product Type

Rat Macrophages

Specification

Cell Source

Liver

Marker expression

Expressed on macrophages, CD11b plays a critical role in regulating pathogen recognition, phagocytosis, and cell survival. The expression of CD11b is positive by flow cytometry.

Status

Frozen

Formulation

Cryopreservation medium

Quality Control

Cells are negative for mycoplasma, bacteria, fungi and yeast.

Medium

Recommended medium

Shipping Info

Dry ice

Size

1 x 10^6 cells, 2 x 10^6 cells, 5 x 10^6 cells, 10 x 10^6 cells

Handling Notes

Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.

Notes

Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

Can these cells reliably mimic Kupffer cell behavior in drug-induced liver injury studies?

Yes. These liver-derived macrophages act as a robust in vitro surrogate for Kupffer cells. They sense DAMPs and PAMPs, respond strongly to endotoxin and hepatotoxic compounds, and release key mediators such as TNF-α, IL-1β, and IL-6.

What viability and morphology can I expect after thawing, and how critical is the thawing protocol?

When the recommended rapid-thaw procedure is followed, post-thaw viability typically exceeds 90%, and cells attach and spread within several hours. Deviations such as slow thawing, prolonged exposure to DMSO, or delayed seeding can reduce viability and impair adherence. We supply a detailed thawing and recovery protocol, including suggested centrifugation steps and seeding densities, to help you maintain optimal morphology and function for both short- and multi-day experiments.

Are specific surface coatings or cultureware types recommended for these liver macrophages?

The cells attach well to standard tissue-culture-treated plastic, which is sufficient for most ELISA, qPCR, and cytokine-release assays. For high-content imaging, long-term cultures, or frequent washing steps, we recommend collagen, fibronectin, or similar ECM coatings to enhance adherence and spreading.

Having a dependable, ready-to-use cell source significantly shortened our assay development timeline
We needed an in vitro liver immune model for early screening. These macrophages gave strong TNF-α and IL-6 readouts and tolerated multiple stimulations in longer time-course experiments.
The overall experience was smooth
The macrophages behaved as expected, showed clean phagocytosis activity, and maintained their phenotype across our experimental runs. This product has become a regular part of our hepatic immunology workflow.
We combined these liver macrophages with primary hepatocytes in a co-culture system
The interaction was very robust, and we could clearly see immune-mediated changes in hepatic readouts. The high viability and responsiveness made data interpretation much more straightforward compared to our previous models.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.