Rat Macrophages, Bone Marrow, 1-6 x 10^6 (MTS-0922-JF96)

Yes. Bone marrow-derived macrophages are highly responsive to classical (e.g., LPS + IFN-γ) and alternative (e.g., IL-4/IL-13) polarization stimuli. When cultured in our recommended M1 or M2 macrophage generation media, they show clear changes in marker expression and cytokine profiles.

Absolutely. The cells adhere well to standard tissue-culture plates and glass-bottom dishes, spreading into a morphology that is very compatible with phase-contrast and fluorescence imaging. They tolerate fixation and staining procedures and have been used successfully in high-content screening formats. For live-cell imaging, we recommend optimizing cell density and using appropriate imaging-compatible media to maintain stable conditions during prolonged recording.

As a starting point, we recommend a seeding density of 1-2 × 10^5 cells/cm^², which typically provides a well-distributed monolayer within 24 hours. For short-term assays such as acute cytokine release, 24-48 hours post-seeding is usually sufficient. For polarization, multi-step stimulation, or long-term exposure to compounds, planning a 3-5 day culture period with regular medium changes will support stable and reproducible results.