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Muscularis Macrophages, Gut (MT-0224-HM19)

Datasheet

Overview

Description

Muscularis macrophages are a specialized subset of macrophages located within the muscularis externa layer of the gastrointestinal tract, particularly prominent in the stomach and intestines. They reside within the smooth muscle layers that surround the gut, forming a network that spans the length of the gastrointestinal tract.

Applications

Numerous types of experimental manipulations, including morphological, gene expression, and physiological studies.

Species

Mouse

Product Type

Tissue-specific Macrophages

Specification

Cell Source

Gut

Status

Frozen

Formulation

Cryopreservation medium

Quality Control

Cells are negative for mycoplasma, bacteria, fungi and yeast.

Medium

Recommended medium

Shipping Info

Dry ice

Size

1×10⁶ cells

Handling Notes

Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.

Storage

Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

How do you confirm the identity and functional competence of the muscularis macrophages before shipping?

Each batch undergoes phenotypic characterization using macrophage markers and transcripts associated with muscularis specialization. We also assess viability, morphology, and responsiveness to defined cytokine stimuli.

Do the macrophages maintain their neuromodulatory phenotype over several days in culture?

Under the recommended conditions, they usually maintain stable viability and functional responsiveness for multiple days. While any primary or specialized cells can undergo some adaptation in vitro, we have optimized the culture guidelines to preserve their neuromodulatory and tissue-associated properties for typical experimental durations. Customers can further tailor media and stimulation regimens to align with specific project needs.

Is large-scale production available for extended studies on gut motility or enteric neuro-immune regulation?

Yes. We offer scalable production and the possibility to reserve multiple batches from the same production series, which supports longitudinal or multi-phase projects.

The muscularis macrophages became a cornerstone of our enteric neuro-immune model
We co-cultured them with enteric neurons and smooth muscle cells, and the resulting system captured many of the regulatory features reported in vivo. Their responses to inflammatory and neuromodulatory cues were clear and reproducible, allowing us to make confident conclusions about signaling pathways influencing gut motility.
The support team was responsive when we had questions about co-culture setup, which helped us avoid common pitfalls and improve experimental success rates
The ability to place these macrophages into engineered tissue constructs opened new possibilities for our group. We could finally examine how targeted interventions influence immune-muscle-nerve interactions in a controlled environment.
We ordered larger batches to support a multi-month motility project, and the batch-to-batch variability was pleasantly low
The macrophages behaved predictably in our functional assays, which made it easier to compare data generated at different times. Overall, this product has proven to be a reliable and scientifically robust tool for exploring intestinal neuro-immune mechanisms.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.