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MacroCargo™ Mouse J774 with Anti-GITR antibody (Viral System, Lentivirus) (MTS-1122-YF495)

Datasheet

Cell Properties

Product Overview

As a therapeutic tool, macrophage cell has a great capacity for delivering cargos because of their intrinsic characteristics. This product is engineered Mouse J774 carried with Anti-GITR antibody by Viral System-Lentivirus. MacroCargo™ products aim to improve the macrophage function and delivery of specific cargos. We also provide custom macrophage delivery systems based on your specific requirements.

Cell Name

J774

Cell Type

Cell Line

Cell Species

Mouse

Cell Background

Mouse mononuclear macrophages J774A.1 is a cell line isolated in 1968 from the ascites of an adult, female mouse with reticulum cell sarcoma. This cell line can be used in immunology research.

Cargo Properties

Cargo Type

Checkpoint antibody

Specific Cargo

Anti-GITR antibody

Target Common Name

TNFRSF18

Target Alternative Names

AITR; GITR; CD357; GITR-D; ENERGEN

Target Full Name

TNF receptor superfamily member 18

Introduction

This gene encodes a member of the TNF-receptor superfamily. The encoded receptor has been shown to have increased expression upon T-cell activation, and it is thought to play a key role in dominant immunological self-tolerance maintained by CD25(+)CD4(+) regulatory T cells. Knockout studies in mice also suggest the role of this receptor is in the regulation of CD3-driven T-cell activation and programmed cell death. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.

UniprotID

Q9Y5U5

GeneID

8784

Cargo Delivery System Type

Viral System

Cargo Delivery Approach

Lentivirus

Product Properties

Applications

Improve macrophages persist in solid tumors

References

Brom, Victoria C et al. "The Role of Immune Checkpoint Molecules on Macrophages in Cancer, Infection, and Autoimmune Pathologies." Frontiers in immunology vol. 13 837645. 28 Mar. 2022, doi:10.3389/fimmu.2022.837645

Mycoplasma Testing

Negative

Sterility Testing

Negative

Shipping

Dry ice

Storage

Frozen cells should be stored in a liquid nitrogen tank (-150°C~-190°C) for long term.

Handling Notes

Frozen cells should be thawed immediately upon receipt and grown according to handling procedure to ensure cell viability and proper assay performance.
Note: Do not freeze the cells upon receipt as it may result in irreversible damage to the cell line.
Disclaimer: We cannot guarantee cell viability if the cells are not thawed immediately upon receipt and grown according to handling procedure.

Restriction

Research use only