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MacroCargo™ Human PBMC-derived Monocytes with IFN-α (Viral System, Lentivirus) (MTS-1222-YF483)

Datasheet

Cell Properties

Product Overview

As a therapeutic tool, macrophage cell has a great capacity for delivering cargos because of their intrinsic characteristics. This product is engineered Human PBMC-derived Monocytes carried with IFN-α by Viral System-Lentivirus. MacroCargo™ products aim to improve the macrophage function and delivery of specific cargos. We also provide custom macrophage delivery systems based on your specific requirements.

Cell Name

PBMC-derived Monocytes

Cell Type

Primary Cell

Cell Species

Human

Cell Background

Monocytes express various receptors, which monitor and sense environmental changes. Monocytes are highly plastic and heterogeneous, and change their functional phenotype in response to environmental stimulation. Evidence from murine and human studies has suggested that monocytosis can be an indicator of various inflammatory diseases. Monocytes can differentiate into inflammatory or anti-inflammatory subsets. Upon tissue damage or infection, monocytes are rapidly recruited to the tissue, where they can differentiate into tissue macrophages or dendritic cells.

Cargo Properties

Cargo Type

Cytokine

Specific Cargo

IFN-α

Cargo Common Name

IFNA1

Cargo Alternative Names

IFL; IFN; IFNA@; IFNA13; leIF D; IFN-ALPHA; IFN-alphaD

Cargo Full Name

Interferon alpha 1

Introduction

This gene is a member of the alpha interferon gene cluster on chromosome 9. The encoded cytokine is a member of the type I interferon family that is produced in response to viral infection as a key part of the innate immune response with potent antiviral, antiproliferative and immunomodulatory properties. This cytokine, like other type I interferons, binds a plasma membrane receptor made of IFNAR1 and IFNAR2 that is ubiquitously expressed, and thus is able to act on virtually all body cells. This cytokine is upregulated in preeclamptic placentas and is thought to be a mediator of preeclampsia.

UniprotID

P01562

GeneID

3439,3447

Cargo Delivery System Type

Viral System

Cargo Delivery Approach

Lentivirus

Product Properties

Applications

Positive regulator of M1 polarization of macrophage; Improve survive; Resistant to reprogramming by tumor-secreted signals; Promote persistence and activation of endogenous or adoptively transferred NK or T cells

References

De Palma, Michele et al. "Tumor-targeted interferon-alpha delivery by Tie2-expressing monocytes inhibits tumor growth and metastasis." Cancer cell vol. 14,4 (2008): 299-311. doi:10.1016/j.ccr.2008.09.004

Mycoplasma Testing

Negative

Sterility Testing

Negative

Shipping

Dry ice

Storage

Frozen cells should be stored in a liquid nitrogen tank (-150°C~-190°C) for long term.

Handling Notes

Frozen cells should be thawed immediately upon receipt and grown according to handling procedure to ensure cell viability and proper assay performance.
Note: Do not freeze the cells upon receipt as it may result in irreversible damage to the cell line.
Disclaimer: We cannot guarantee cell viability if the cells are not thawed immediately upon receipt and grown according to handling procedure.

Restriction

Research use only