Reprogramming Tumor-associated Macrophages (TAMs) by Targeted mRNA Nanoparticles

More and more scientific evidence indicates that tumor-associated macrophages (TAMs) play a crucial role in tumor development and progression. TAMs influence tumor development by inducing immunosuppression. With our state-of-the-art technology and industry-leading expertise, Creative Biolabs has developed an unparalleled platform Macrophage Therapeutics Development Platform for reprogramming TAMs to M1 phenotype. We are committed to providing overall solutions for TAM reprogramming using targeted mRNA nanoparticles, focusing on innovative research.

Background of Reprogramming TAMs to Tumor-clearing M1 Phenotype

The high density of TAMs correlates with poor prognosis and reduced overall patient survival. Studies have shown that cytokines and chemokines, secreted through cross-talk between TAMs and tumor cells, strongly induce a hyperproliferative, antiapoptotic, and metastatic response in several cancers. This finding offers a potential therapeutic option for treating cancers. However, TAMs exhibit functional heterogeneity ranging from immune-activated, proinflammatory, and antitumor responses to regulatory, anti-inflammatory, and protumoral activities. TAMs usually express an M2 phenotype, which enables them to perform immunosuppressive and tumor-promoting functions. Reprogramming these TAMs toward a tumor-clearing M1 phenotype could thwart their pro-cancer activities and unleash anti-tumor immunity.

Reprogramming TAM by Targeted mRNA Nanoparticles at Creative Biolabs

In vitro-transcribed (IVT) mRNA is an ideal carrier to deliver genetic information into living cells and many techniques of IVT mRNA have shown to be useful for many applications, such as targeted mRNA nanoparticles, which have gained broad attention in the reprogramming of TAMs.

Fig.1 Schematic illustration of polymeric nanoparticles. (Sarode, 2020)

Creative Biolabs has organized a staff of outstanding scientists who have engaged in TAM reprogramming for many years. Our scientists have developed an efficient strategy for TAM reprogramming using targeted mRNA nanoparticles. The suppressive M2 macrophages can be reprogrammed to M1 phenotype by targeted nanoparticles, which contain mRNA encoding master regulators of macrophage polarization, such as interferon-regulatory factors (IRF) family, as β-catenin, or FOS-like antigen 2 (FOSL2). To ensure intracellular stability, translational efficiency, and low immunogenicity, Nanoparticle-delivered IVT mRNA constructs have been designed with structural modifications, which have been shown to improve cytosolic persistence and protein expression. Creative Biolabs has perfected our technical pipelines in reprogramming TAMs by targeted mRNA nanoparticles. We would like to share our knowledge and passion in this field to facilitate our clients' research and project development.

Creative Biolabs is committed to providing overall solutions for TAM reprogramming. Our team has extensive experience in macrophage development and provides accurate and effective solutions for researchers all over the world. For more detailed information on our TAM reprogramming service, please feel free to contact us and further discuss it with our scientists.

Reference

1. Sarode, P.; et al. Reprogramming of tumor-associated macrophages by targeting β-catenin/FOSL2/ARID5A signaling: A potential treatment of lung cancer. Science advances. 2020, 6(23):eaaz6105.