Macrophages, vital components of the mononuclear phagocyte system, serve pivotal functions in prenatal development, upkeep of tissue stability, and the progression of diseases. Significant efforts have been invested in constructing genetic models to deplete macrophages in mice, facilitating investigations into the precise functions of tissue-specific macrophages in physiological and pathological contexts. However, the complex heterogeneity within macrophages has hindered the development of an ideal genetic depletion model for these cells as of yet. As a result, Creative Biolabs has established a strategic platform for developing mouse models with macrophage depletion to further explore the intricate mechanisms of macrophages in different environments.

Fig.1 Macrophage depletion is crucial for enhancing the interaction between TAMs and CD8⁺ T cells.¹

Macrophage Depletion Mouse Model Development Service at Creative Biolabs

Creative Biolabs' macrophage depletion mouse model development service combines a variety of advanced technologies and cutting-edge theoretical knowledge, and strives to develop more precise macrophage depletion mouse models that are beneficial to disease research.

We offer a variety of tailored macrophage depletion models dedicated to advancing therapeutic research projects. These models utilize specific markers to achieve targeted macrophage depletion either in tissues or throughout the body. By identifying key transcription factors and surface markers of macrophage subsets in diverse tissues, we facilitate the creation of more precise tools for genetic macrophage depletion. Additionally, we have the capability to customize macrophage depletion mouse models based on your specific requirements. Creative Biolabs is your trusted partner for achieving successful outcomes in a global context.

Available Macrophage Depletion Models at Creative Biolabs

• Macrophage Fas-induced apoptosis (MAFIA) transgenic mice: This model enables reversible depletion of macrophages using an inducible genetic system targeting the mouse Csf1r gene. It is valuable for studying macrophage functions in various diseases and tissue processes.
• CD11b-DTR transgenic mice: This model employs a diphtheria toxin (DT) inducible system with the human CD11b promoter driving the human DT receptor fusion. Widely used for studying macrophage biology in tissue homeostasis and disease contexts.
• CD169-DTR strain: The CD169-DTR strain features a sophisticated DT-inducible system with the human DTR controlled by the CD169 sialoadhesin gene promoter. This strain allows for precise conditional ablation of macrophages in specific regions such as the spleen's marginal metallophilic zone, lymph nodes, and bone marrow.
• CD206-DTR mice: The CD206-DTR strain was developed to study the specific roles of M2 macrophages in mice. It provides a valuable tool for investigating the unique functions of both M1 and M2 macrophages in different disease settings.

Creative Biolabs is at the forefront of tissue-specific macrophage research, offering cutting-edge technologies and services to support global customers in advancing their therapeutic development. Contact us for expert assistance in developing macrophage depletion mouse models.