Macrophage Culture

Culturing macrophages in vitro provides a valuable tool for studying their behavior and interactions. In this section, Creative Biolabs aims to provide protocols related to macrophage culture, which provide researchers with a step-by-step guide for successfully establishing and maintaining primary macrophage cultures, allowing for reproducible and reliable experimental outcomes.

Click on the items below to find protocols related to macrophage culture.

Fig.1 Human monocyte-derived macrophages culturing. (Creative Biolabs Original)

Culture of Human Monocyte-Derived Macrophages

Fig.2 Murine bone marrow-derived macrophages culturing. (Creative Biolabs Original)

Culture of Murine Bone Marrow-Derived Macrophages

Fig.3 Differentiation of hiPSCs into functional macrophages. (Creative Biolabs Original)

Differentiation of Human Induced Pluripotent Stem Cells into Functional Macrophages

Fig.4 Coculturing of macrophages with other cells. (Creative Biolabs Original)

Macrophage Co-Culture

Materials

Specialized culture media
Such as RPMI-1640 or DMEM, are often supplemented with fetal bovine serum (FBS), cytokines, and growth factors to promote macrophage survival, proliferation, and differentiation.
Specific stimuli
Such as lipopolysaccharide (LPS) or interferon-gamma (IFN-γ), can trigger macrophage activation, leading to a spectrum of functional phenotypes.

Applications of Macrophage Culture

Infectious Disease Research: Researchers can employ macrophage culture to investigate host-pathogen interactions, study virulence factors, and develop novel therapeutics against infectious diseases.
Immunotherapy and Cancer Research: Researchers can leverage macrophage culture to develop innovative immunotherapies. This includes macrophage-based adoptive cell therapy, immunomodulatory drug discovery, and targeted delivery systems, revolutionizing the fight against cancer.
Tissue Engineering and Regenerative Medicine: Through macrophage culture, researchers can explore their potential in tissue engineering, biomaterial testing, and the development of regenerative therapies, aiming to restore function and enhance healing in damaged tissues.

Culturing macrophages is a delicate balance between replicating their natural environment and providing the necessary growth factors and nutrients. Creative Biolabs employs cutting-edge techniques and optimized protocols to cultivate macrophages from various sources, including primary cells, cell lines, and induced pluripotent stem cells (iPSCs).