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MacroCargo™ Mouse J774 with Nos2 (Viral System, Lentivirus) (MTS-1122-YF488)

Datasheet

Cell Properties

Product Overview

As a therapeutic tool, macrophage cell has a great capacity for delivering cargos because of their intrinsic characteristics. This product is engineered Mouse J774 carried with Nos2 by Viral System-Lentivirus. MacroCargo™ products aim to improve the macrophage function and delivery of specific cargos. We also provide custom macrophage delivery systems based on your specific requirements.

Cell Name

J774

Cell Type

Cell Line

Cell Species

Mouse

Cell Background

Mouse mononuclear macrophages J774A.1 is a cell line isolated in 1968 from the ascites of an adult, female mouse with reticulum cell sarcoma. This cell line can be used in immunology research.

Cargo Properties

Cargo Type

Protein

Specific Cargo

Nos2

Cargo Common Name

NOS2

Cargo Alternative Names

NOS; INOS; NOS2A; HEP-NOS

Cargo Full Name

Nitric oxide synthase 2

Introduction

Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. Three related pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17.

UniprotID

P35228

GeneID

4843

Cargo Delivery System Type

Viral System

Cargo Delivery Approach

Lentivirus

Product Properties

Applications

M1 macrophage activation; Promoting a T cell response

References

Brom, Victoria C et al. "The Role of Immune Checkpoint Molecules on Macrophages in Cancer, Infection, and Autoimmune Pathologies." Frontiers in immunology vol. 13 837645. 28 Mar. 2022, doi:10.3389/fimmu.2022.837645

Mycoplasma Testing

Negative

Sterility Testing

Negative

Shipping

Dry ice

Storage

Frozen cells should be stored in a liquid nitrogen tank (-150°C~-190°C) for long term.

Handling Notes

Frozen cells should be thawed immediately upon receipt and grown according to handling procedure to ensure cell viability and proper assay performance.
Note: Do not freeze the cells upon receipt as it may result in irreversible damage to the cell line.
Disclaimer: We cannot guarantee cell viability if the cells are not thawed immediately upon receipt and grown according to handling procedure.

Restriction

Research use only