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MacroCargo™ Mouse J774 with IL-12 (Viral System, Lentivirus) (MTS-1122-YF187)

Datasheet

Cell Properties

Product Overview

As a therapeutic tool, macrophage cell has a great capacity for delivering cargos because of their intrinsic characteristics. This product is engineered Mouse J774 carried with IL-12 by Viral System-Lentivirus. MacroCargo™ products aim to improve the macrophage function and delivery of specific cargos. We also provide custom macrophage delivery systems based on your specific requirements.

Cell Name

J774

Cell Type

Cell Line

Cell Species

Mouse

Cell Background

Mouse mononuclear macrophages J774A.1 is a cell line isolated in 1968 from the ascites of an adult, female mouse with reticulum cell sarcoma. This cell line can be used in immunology research.

Cargo Properties

Cargo Type

Cytokine

Specific Cargo

IL-12

Cargo Common Name

IL12

Cargo Alternative Names

IL12; IL-12; Interleukin 12

Cargo Full Name

Interleukin 12

Introduction

Interleukin-12 (IL-12) is a heterodimeric cytokine produced mostly by phagocytic cells in response to bacteria, bacterial products, and intracellular parasites, and to some degree by B lymphocytes. IL-12 induces cytokine production, primarily of IFN-gamma, from NK and T cells, acts as a growth factor for activated NK and T cells, enhances the cytotoxic activity of NK cells, and favors cytotoxic T lymphocyte generation.

Cargo Delivery System Type

Viral System

Cargo Delivery Approach

Lentivirus

Product Properties

Applications

Positive regulator of M1 polarization of macrophage; Improve survive; Promote persistence and activation of endogenous or adoptively transferred NK or T cells

References

Brempelis, Katherine J et al. "Genetically engineered macrophages persist in solid tumors and locally deliver therapeutic proteins to activate immune responses." Journal for immunotherapy of cancer vol. 8,2 (2020): e001356. doi:10.1136/jitc-2020-001356

Mycoplasma Testing

Negative

Sterility Testing

Negative

Shipping

Dry ice

Storage

Frozen cells should be stored in a liquid nitrogen tank (-150°C~-190°C) for long term.

Handling Notes

Frozen cells should be thawed immediately upon receipt and grown according to handling procedure to ensure cell viability and proper assay performance.
Note: Do not freeze the cells upon receipt as it may result in irreversible damage to the cell line.
Disclaimer: We cannot guarantee cell viability if the cells are not thawed immediately upon receipt and grown according to handling procedure.

Restriction

Research use only