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MacroCargo™ Mouse Bone Marrow-derived Hematopoietic Stem/Progenitor Cells (HSPCs) with GDNF (Transfection System, GenePORTER 3000) (MTS-1122-YF118)

Datasheet

Cell Properties

Product Overview

As a therapeutic tool, macrophage cell has a great capacity for delivering cargos because of their intrinsic characteristics. This product is engineered Mouse Bone Marrow-derived Hematopoietic Stem/Progenitor Cells (HSPCs) carried with GDNF by Transfection System-GenePORTER 3000. MacroCargo™ products aim to improve the macrophage function and delivery of specific cargos. We also provide custom macrophage delivery systems based on your specific requirements.

Cell Name

Bone Marrow-derived Hematopoietic Stem/Progenitor Cells (HSPCs)

Cell Type

Primary Cell

Cell Species

Mouse

Cell Background

High-dose chemotherapy followed by adjuvant autologous hematopoietic stem/progenitor cell (HSPC) transplantation has been tested for the treatment of hematological and solid tumors and can be regarded as a clinical option for certain types of cancer. Scientists have highlighted the important contribution of bone marrow (BM)-derived myeloid cells to tumor angiogenesis.

Cargo Properties

Cargo Type

Protein

Specific Cargo

GDNF

Cargo Common Name

GDNF

Cargo Alternative Names

ATF; ATF1; ATF2; HSCR3; HFB1-GDNF

Cargo Full Name

Glial cell derived neurotrophic factor

Introduction

This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. The recombinant form of this protein, a highly conserved neurotrophic factor, was shown to promote the survival and differentiation of dopaminergic neurons in culture, and was able to prevent apoptosis of motor neurons induced by axotomy. This protein is a ligand for the product of the RET (rearranged during transfection) protooncogene. Mutations in this gene may be associated with Hirschsprung disease and Tourette syndrome. This gene encodes multiple protein isoforms that may undergo similar proteolytic processing.

UniprotID

P39905

GeneID

2668

Cargo Delivery System Type

Transfection System

Cargo Delivery Approach

GenePORTER 3000

Product Properties

Applications

Provide significant neuronal protection in Parkinson's disease

References

Zhao, Yuling et al. "GDNF-transfected macrophages produce potent neuroprotective effects in Parkinson's disease mouse model." PloS one vol. 9,9 e106867. 17 Sep. 2014, doi:10.1371/journal.pone.0106867

Mycoplasma Testing

Negative

Sterility Testing

Negative

Shipping

Dry ice

Storage

Frozen cells should be stored in a liquid nitrogen tank (-150°C~-190°C) for long term.

Handling Notes

Frozen cells should be thawed immediately upon receipt and grown according to handling procedure to ensure cell viability and proper assay performance.
Note: Do not freeze the cells upon receipt as it may result in irreversible damage to the cell line.
Disclaimer: We cannot guarantee cell viability if the cells are not thawed immediately upon receipt and grown according to handling procedure.

Restriction

Research use only