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MacroCargo™ Human Monocyte-derived Macrophages (MDMs) with B7-H4 gRNA (Viral System, lentivirus) (MTS-1122-YF314)

Datasheet

Cell Properties

Product Overview

As a therapeutic tool, macrophage cell has a great capacity for delivering cargos because of their intrinsic characteristics. This product is engineered Human Monocyte-derived Macrophages (MDMs) carried with B7-H4 gRNA by Viral System-lentivirus. MacroCargo™ products aim to improve the macrophage function and delivery of specific cargos. We also provide custom macrophage delivery systems based on your specific requirements.

Cell Name

Monocyte-derived Macrophages (MDMs)

Cell Type

Primary Cell

Cell Species

Human

Cell Background

The culture of human monocyte-derived macrophages (MDMs) represents a tool to study macrophages, with monocytes known to give rise to tissue macrophages influenced by certain environmental cues.

Cargo Properties

Cargo Type

CRISPR KO

Specific Cargo

B7-H4 gRNA

Target Common Name

VTCN1

Target Alternative Names

B7X; B7H4; B7S1; B7-H4; B7h.5; VCTN1; PRO1291

Target Full Name

V-set domain containing T cell activation inhibitor 1

Introduction

This gene encodes a protein belonging to the B7 costimulatory protein family. Proteins in this family are present on the surface of antigen-presenting cells and interact with ligand bound to receptors on the surface of T cells. Studies have shown that high levels of the encoded protein has been correlated with tumor progression. A pseudogene of this gene is located on chromosome 20. Multiple transcript variants encoding different isoforms have been found for this gene.

UniprotID

Q7Z7D3

GeneID

79679

Cargo Delivery System Type

Viral System

Cargo Delivery Approach

Lentivirus

Product Properties

Applications

Prevent cancer cells from evading immune clearance

References

Brom, Victoria C et al. "The Role of Immune Checkpoint Molecules on Macrophages in Cancer, Infection, and Autoimmune Pathologies." Frontiers in immunology vol. 13 837645. 28 Mar. 2022, doi:10.3389/fimmu.2022.837645

Mycoplasma Testing

Negative

Sterility Testing

Negative

Shipping

Dry ice

Storage

Frozen cells should be stored in a liquid nitrogen tank (-150°C~-190°C) for long term.

Handling Notes

Frozen cells should be thawed immediately upon receipt and grown according to handling procedure to ensure cell viability and proper assay performance.
Note: Do not freeze the cells upon receipt as it may result in irreversible damage to the cell line.
Disclaimer: We cannot guarantee cell viability if the cells are not thawed immediately upon receipt and grown according to handling procedure.

Restriction

Research use only