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MacroCargo™ Mouse J774 with SIGLEC1 gRNA (Viral System, Adenovirus) (MTS-1122-YF481)

Datasheet

Cell Properties

Product Overview

As a therapeutic tool, macrophage cell has a great capacity for delivering cargos because of their intrinsic characteristics. This product is engineered Mouse J774 carried with SIGLEC1 gRNA by Viral System-Adenovirus. MacroCargo™ products aim to improve the macrophage function and delivery of specific cargos. We also provide custom macrophage delivery systems based on your specific requirements.

Cell Name

J774

Cell Type

Cell Line

Cell Species

Mouse

Cell Background

Mouse mononuclear macrophages J774A.1 is a cell line isolated in 1968 from the ascites of an adult, female mouse with reticulum cell sarcoma. This cell line can be used in immunology research.

Cargo Properties

Cargo Type

CRISPR KO

Specific Cargo

SIGLEC1 gRNA

Target Common Name

SIGLEC1

Target Alternative Names

SN; CD169; SIGLEC-1

Target Full Name

Sialic acid binding Ig like lectin 1

Introduction

This gene encodes a member of the immunoglobulin superfamily. The encoded protein is a lectin-like adhesion molecule that binds glycoconjugate ligands on cell surfaces in a sialic acid-dependent manner. It is a type I transmembrane protein expressed only by a subpopulation of macrophages and is involved in mediating cell-cell interactions. The protein plays an important role in multiple human diseases and bacterial and viral infections has been shown to enhance SARS-CoV-2 infection.

UniprotID

Q9BZZ2

GeneID

6614

Cargo Delivery System Type

Viral System

Cargo Delivery Approach

Adenovirus

Product Properties

Applications

Promote the macrophage capability to limit tumor growth

References

Mantovani, Alberto et al. "Macrophages as tools and targets in cancer therapy." Nature reviews. Drug discovery vol. 21,11 (2022): 799-820. doi:10.1038/s41573-022-00520-5

Mycoplasma Testing

Negative

Sterility Testing

Negative

Shipping

Dry ice

Storage

Frozen cells should be stored in a liquid nitrogen tank (-150°C~-190°C) for long term.

Handling Notes

Frozen cells should be thawed immediately upon receipt and grown according to handling procedure to ensure cell viability and proper assay performance.
Note: Do not freeze the cells upon receipt as it may result in irreversible damage to the cell line.
Disclaimer: We cannot guarantee cell viability if the cells are not thawed immediately upon receipt and grown according to handling procedure.

Restriction

Research use only