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MacroCargo™ Mouse Bone Marrow-derived Macrophages (BMDM) with pDNA encoding IL-5 (Nanoparticle System, Magnetic cationic liposomes) (MTS-1222-YF121)

Datasheet

Cell Properties

Product Overview

As a therapeutic tool, macrophage cell has a great capacity for delivering cargos because of their intrinsic characteristics. This product is engineered Mouse Bone Marrow-derived Macrophages (BMDM) carried with pDNA encoding IL-5 by Nanoparticle System-Magnetic cationic liposomes. MacroCargo™ products aim to improve the macrophage function and delivery of specific cargos. We also provide custom macrophage delivery systems based on your specific requirements.

Cell Name

Bone Marrow-derived Macrophages (BMDM)

Cell Type

Primary Cell

Cell Species

Mouse

Cell Background

The broad use of transgenic and gene-targeted mice has established bone marrow-derived macrophages (BMDM) as important mammalian host cells for investigation of the macrophages biology.

Cargo Properties

Cargo Type

Cytokine

Specific Cargo

pDNA encoding IL-5

Cargo Common Name

IL5

Cargo Alternative Names

EDF; TRF; IL-5

Cargo Full Name

Interleukin 5

Introduction

This gene encodes a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. The encoded cytokine plays a major role in the regulation of eosinophil formation, maturation, recruitment and survival. The increased production of this cytokine may be related to pathogenesis of eosinophil-dependent inflammatory diseases. This cytokine functions by binding to its receptor, which is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF). This gene is located on chromosome 5 within a cytokine gene cluster which includes interleukin 4 (IL4), interleukin 13 (IL13), and CSF2 . This gene, IL4, and IL13 may be regulated coordinately by long-range regulatory elements spread over 120 kilobases on chromosome 5q31.

UniprotID

P05113

GeneID

3567

Cargo Delivery System Type

Nanoparticle System

Cargo Delivery Approach

Magnetic cationic liposomes

Nanoparticle Component

DOTAP, DSPC, cholesterol, iron oxide (II, III).

Product Properties

Applications

Improve the delivery of macrophages to tumors and its therapeutic efficacy against inflammatory diseases

Mycoplasma Testing

Negative

Sterility Testing

Negative

Shipping

Dry ice

Storage

Frozen cells should be stored in a liquid nitrogen tank (-150°C~-190°C) for long term.

Handling Notes

Frozen cells should be thawed immediately upon receipt and grown according to handling procedure to ensure cell viability and proper assay performance.
Note: Do not freeze the cells upon receipt as it may result in irreversible damage to the cell line.
Disclaimer: We cannot guarantee cell viability if the cells are not thawed immediately upon receipt and grown according to handling procedure.

Restriction

Research use only