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MacroCargo™ Human PBMC-derived Monocytes with PD-L1 gRNA (Viral System, Lentivirus) (MTS-1222-YF496)

Datasheet

Cell Properties

Product Overview

As a therapeutic tool, macrophage cell has a great capacity for delivering cargos because of their intrinsic characteristics. This product is engineered Human PBMC-derived Monocytes carried with PD-L1 gRNA by Viral System-Lentivirus. MacroCargo™ products aim to improve the macrophage function and delivery of specific cargos. We also provide custom macrophage delivery systems based on your specific requirements.

Cell Name

PBMC-derived Monocytes

Cell Type

Primary Cell

Cell Species

Human

Cell Background

Monocytes express various receptors, which monitor and sense environmental changes. Monocytes are highly plastic and heterogeneous, and change their functional phenotype in response to environmental stimulation. Evidence from murine and human studies has suggested that monocytosis can be an indicator of various inflammatory diseases. Monocytes can differentiate into inflammatory or anti-inflammatory subsets. Upon tissue damage or infection, monocytes are rapidly recruited to the tissue, where they can differentiate into tissue macrophages or dendritic cells.

Cargo Properties

Cargo Type

CRISPR KO

Specific Cargo

PD-L1 gRNA

Target Common Name

CD274

Target Alternative Names

B7-H; B7H1; PDL1; PD-L1; hPD-L1; PDCD1L1; PDCD1LG1

Target Full Name

CD274 molecule

Introduction

This gene encodes an immune inhibitory receptor ligand that is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. The encoded protein is a type I transmembrane protein that has immunoglobulin V-like and C-like domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation. Expression of this gene in tumor cells is considered to be prognostic in many types of human malignancies, including colon cancer and renal cell carcinoma. Alternative splicing results in multiple transcript variants.

UniprotID

Q9NZQ7

GeneID

29126

Cargo Delivery System Type

Viral System

Cargo Delivery Approach

Lentivirus

Product Properties

Applications

Resistant to reprogramming by tumor-secreted signals

References

Moyes, Kara W et al. "Genetically Engineered Macrophages: A Potential Platform for Cancer Immunotherapy." Human gene therapy vol. 28,2 (2017): 200-215. doi:10.1089/hum.2016.060

Mycoplasma Testing

Negative

Sterility Testing

Negative

Shipping

Dry ice

Storage

Frozen cells should be stored in a liquid nitrogen tank (-150°C~-190°C) for long term.

Handling Notes

Frozen cells should be thawed immediately upon receipt and grown according to handling procedure to ensure cell viability and proper assay performance.
Note: Do not freeze the cells upon receipt as it may result in irreversible damage to the cell line.
Disclaimer: We cannot guarantee cell viability if the cells are not thawed immediately upon receipt and grown according to handling procedure.

Restriction

Research use only