CSF-1R gRNA Lentiviral Particle for Macrophage Engineering (MTS-0124-LX29)

Engineering Approach

Target KO gRNA Contruction Lentivirus

Species

Human

Target

CSF-1R gRNA

Alternative Names

FMS; CSFR; FIM2; HDLS; C-FMS; CD115; HDLS1; CSF-1R; BANDDOS; M-CSF-R

Full Name

Colony stimulating factor 1 receptor

Introduction

The protein encoded by this gene is the receptor for colony stimulating factor 1, a cytokine which controls the production, differentiation, and function of macrophages. This receptor mediates most if not all of the biological effects of this cytokine. Ligand binding activates the receptor kinase through a process of oligomerization and transphosphorylation. The encoded protein is a tyrosine kinase transmembrane receptor and member of the CSF1/PDGF receptor family of tyrosine-protein kinases. Mutations in this gene have been associated with a predisposition to myeloid malignancy. The first intron of this gene contains a transcriptionally inactive ribosomal protein L7 processed pseudogene oriented in the opposite direction. Alternative splicing results in multiple transcript variants. Expression of a splice variant from an LTR promoter has been found in Hodgkin lymphoma (HL), HL cell lines and anaplastic large cell lymphoma.

UniprotID

GeneID

Applications

Inhibite tumor growth

Reference

Mantovani, Alberto et al. "Macrophages as tools and targets in cancer therapy." Nature reviews. Drug discovery vol. 21,11 (2022): 799-820. doi:10.1038/s41573-022-00520-5

Packaging System

Lentivirus

Titer

>1E8 TU/mL

Size

200uL; 1mL

Mycoplasma Testing

Negative

Sterility Testing

Negative

Shipping

Dry ice

Storage

Stored at -80°C for long term.

Handling Notes

Avoid multiple freeze/thaw cycles.

Restriction

RUO