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MacroCargo™ Rat Alveolar Macrophages with pDNA encoding TNF-α (Nanoparticle System, Magnetic cationic liposomes) (MTS-1122-YF272)

Datasheet

Cell Properties

Product Overview

As a therapeutic tool, macrophage cell has a great capacity for delivering cargos because of their intrinsic characteristics. This product is engineered Rat Alveolar Macrophages carried with pDNA encoding TNF-α by Nanoparticle System-Magnetic cationic liposomes. MacroCargo™ products aim to improve the macrophage function and delivery of specific cargos. We also provide custom macrophage delivery systems based on your specific requirements.

Cell Name

Rat Alveolar Macrophages

Cell Type

Primary Cell

Cell Species

Rat

Cell Background

Alveolar macrophages (AM) are important in the regulation of immune responses in the lung, through their role as scavenger cells and through the production of many bioactive factors.

Cargo Properties

Cargo Type

Cytokine

Specific Cargo

pDNA encoding TNF-α

Cargo Common Name

TNF

Cargo Alternative Names

DIF; TNFA; TNFSF2; TNLG1F; TNF-alpha

Cargo Full Name

Tumor necrosis factor

Introduction

This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, psoriasis, rheumatoid arthritis ankylosing spondylitis, tuberculosis, autosomal dominant polycystic kidney disease, and cancer. Mutations in this gene affect susceptibility to cerebral malaria, septic shock, and Alzheimer disease. Knockout studies in mice also suggested the neuroprotective function of this cytokine.

UniprotID

P01375

GeneID

7124

Cargo Delivery System Type

Nanoparticle System

Cargo Delivery Approach

Magnetic cationic liposomes

Nanoparticle Component

DOTAP, DSPC, cholesterol, iron oxide (II, III).

Product Properties

Applications

Improve the colonic delivery of macrophages and its therapeutic efficacy against colonic inflammatory diseases.

References

Kono, Yusuke et al. "Enhanced macrophage delivery to the colon using magnetic lipoplexes with a magnetic field." Drug deliveryvol. 26,1 (2019): 935-943. doi:10.1080/10717544.2019.1662515

Mycoplasma Testing

Negative

Sterility Testing

Negative

Shipping

Dry ice

Storage

Frozen cells should be stored in a liquid nitrogen tank (-150°C~-190°C) for long term.

Handling Notes

Frozen cells should be thawed immediately upon receipt and grown according to handling procedure to ensure cell viability and proper assay performance.
Note: Do not freeze the cells upon receipt as it may result in irreversible damage to the cell line.
Disclaimer: We cannot guarantee cell viability if the cells are not thawed immediately upon receipt and grown according to handling procedure.

Restriction

Research use only