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MacroCargo™ Mouse RAW264.7 with Anti-VEGFA antibody (Viral System, Adenovirus) (MTS-1122-YF435)

Cell Properties

Product Overview
As a therapeutic tool, macrophage cell has a great capacity for delivering cargos because of their intrinsic characteristics. This product is engineered Mouse RAW264.7 carried with Anti-VEGFA antibody by Viral System-Adenovirus. MacroCargo™ products aim to improve the macrophage function and delivery of specific cargos. We also provide custom macrophage delivery systems based on your specific requirements.
Cell Name
RAW264.7
Cell Type
Cell Line
Cell Species
Mouse
Cell Background
RAW 264.7 is a macrophage cell line that was established from a tumor in a male mouse induced with the Abelson murine leukemia virus.

Cargo Properties

Cargo Type
Checkpoint antibody
Specific Cargo
Anti-VEGFA antibody
Target Common Name
VEGFA
Target Alternative Names
VPF; VEGF; MVCD1
Target Full Name
Vascular endothelial growth factor A
Introduction
This gene is a member of the PDGF/VEGF growth factor family. It encodes a heparin-binding protein, which exists as a disulfide-linked homodimer. This growth factor induces proliferation and migration of vascular endothelial cells, and is essential for both physiological and pathological angiogenesis. Disruption of this gene in mice resulted in abnormal embryonic blood vessel formation. This gene is upregulated in many known tumors and its expression is correlated with tumor stage and progression. Elevated levels of this protein are found in patients with POEMS syndrome, also known as Crow-Fukase syndrome. Allelic variants of this gene have been associated with microvascular complications of diabetes 1 (MVCD1) and atherosclerosis. Alternatively spliced transcript variants encoding different isoforms have been described. There is also evidence for alternative translation initiation from upstream non-AUG (CUG) codons resulting in additional isoforms. A recent study showed that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is antiangiogenic. Expression of some isoforms derived from the AUG start codon is regulated by a small upstream open reading frame, which is located within an internal ribosome entry site. The levels of VEGF are increased during infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), thus promoting inflammation by facilitating recruitment of inflammatory cells, and by increasing the level of angiopoietin II (Ang II), one of two products of the SARS-CoV-2 binding target, angiotensin-converting enzyme 2 (ACE2). In turn, Ang II facilitates the elevation of VEGF, thus forming a vicious cycle in the release of inflammatory cytokines.
UniprotID
GeneID
Cargo Delivery System Type
Viral System
Cargo Delivery Approach
Adenovirus

Product Properties

Applications
Improve macrophages persist in solid tumors
References
DeNardo, David G, and Brian Ruffell. "Macrophages as regulators of tumour immunity and immunotherapy." Nature reviews. Immunology vol. 19,6 (2019): 369-382. doi:10.1038/s41577-019-0127-6
Mycoplasma Testing
Negative
Sterility Testing
Negative
Shipping
Dry ice
Storage
Frozen cells should be stored in a liquid nitrogen tank (-150°C~-190°C) for long term.
Handling Notes
Frozen cells should be thawed immediately upon receipt and grown according to handling procedure to ensure cell viability and proper assay performance.
Note: Do not freeze the cells upon receipt as it may result in irreversible damage to the cell line.
Disclaimer: We cannot guarantee cell viability if the cells are not thawed immediately upon receipt and grown according to handling procedure.
Restriction
Research use only

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.

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