Online Inquiry

MacroCargo™ Mouse J774 with SIGLEC9 gRNA (Viral System, Lentivirus) (MTS-1122-YF483)

Datasheet

Cell Properties

Product Overview

As a therapeutic tool, macrophage cell has a great capacity for delivering cargos because of their intrinsic characteristics. This product is engineered Mouse J774 carried with SIGLEC9 gRNA by Viral System-Lentivirus. MacroCargo™ products aim to improve the macrophage function and delivery of specific cargos. We also provide custom macrophage delivery systems based on your specific requirements.

Cell Name

J774

Cell Type

Cell Line

Cell Species

Mouse

Cell Background

Mouse mononuclear macrophages J774A.1 is a cell line isolated in 1968 from the ascites of an adult, female mouse with reticulum cell sarcoma. This cell line can be used in immunology research.

Cargo Properties

Cargo Type

CRISPR KO

Specific Cargo

SIGLEC9 gRNA

Target Common Name

SIGLEC9

Target Alternative Names

CD329; CDw329; FOAP-9; siglec-9; OBBP-LIKE

Target Full Name

Sialic acid binding Ig like lectin 9

Introduction

Predicted to enable monosaccharide binding activity and sialic acid binding activity. Predicted to be involved in cell adhesion. Predicted to act upstream of or within negative regulation of inflammatory response and negative regulation of phagocytosis, engulfment. Predicted to be located in external side of plasma membrane. Predicted to be active in plasma membrane.

UniprotID

Q9Y336

GeneID

27180

Cargo Delivery System Type

Viral System

Cargo Delivery Approach

Lentivirus

Product Properties

Applications

Promote the macrophage capability to limit tumor growth

References

Mantovani, Alberto et al. "Macrophages as tools and targets in cancer therapy." Nature reviews. Drug discovery vol. 21,11 (2022): 799-820. doi:10.1038/s41573-022-00520-5

Mycoplasma Testing

Negative

Sterility Testing

Negative

Shipping

Dry ice

Storage

Frozen cells should be stored in a liquid nitrogen tank (-150°C~-190°C) for long term.

Handling Notes

Frozen cells should be thawed immediately upon receipt and grown according to handling procedure to ensure cell viability and proper assay performance.
Note: Do not freeze the cells upon receipt as it may result in irreversible damage to the cell line.
Disclaimer: We cannot guarantee cell viability if the cells are not thawed immediately upon receipt and grown according to handling procedure.

Restriction

Research use only