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MacroCargo™ Mouse J774 with CD73 gRNA (Viral System, Lentivirus) (MTS-1122-YF475)

Datasheet

Cell Properties

Product Overview

As a therapeutic tool, macrophage cell has a great capacity for delivering cargos because of their intrinsic characteristics. This product is engineered Mouse J774 carried with CD73 gRNA by Viral System-Lentivirus. MacroCargo™ products aim to improve the macrophage function and delivery of specific cargos. We also provide custom macrophage delivery systems based on your specific requirements.

Cell Name

J774

Cell Type

Cell Line

Cell Species

Mouse

Cell Background

Mouse mononuclear macrophages J774A.1 is a cell line isolated in 1968 from the ascites of an adult, female mouse with reticulum cell sarcoma. This cell line can be used in immunology research.

Cargo Properties

Cargo Type

CRISPR KO

Specific Cargo

CD73 gRNA

Target Common Name

NT5E

Target Alternative Names

NT; eN; NT5; NTE; eNT; CD73; E5NT; CALJA

Target Full Name

5'-nucleotidase ecto

Introduction

The protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene.

UniprotID

P21589

GeneID

4907

Cargo Delivery System Type

Viral System

Cargo Delivery Approach

Lentivirus

Product Properties

Applications

Prevent cancer cells from evading immune clearance

References

Brom, Victoria C et al. "The Role of Immune Checkpoint Molecules on Macrophages in Cancer, Infection, and Autoimmune Pathologies." Frontiers in immunology vol. 13 837645. 28 Mar. 2022, doi:10.3389/fimmu.2022.837645

Mycoplasma Testing

Negative

Sterility Testing

Negative

Shipping

Dry ice

Storage

Frozen cells should be stored in a liquid nitrogen tank (-150°C~-190°C) for long term.

Handling Notes

Frozen cells should be thawed immediately upon receipt and grown according to handling procedure to ensure cell viability and proper assay performance.
Note: Do not freeze the cells upon receipt as it may result in irreversible damage to the cell line.
Disclaimer: We cannot guarantee cell viability if the cells are not thawed immediately upon receipt and grown according to handling procedure.

Restriction

Research use only