MacroCargo™ Mouse Bone Marrow-derived Hematopoietic Stem/Progenitor Cells (HSPCs) with IL-10 gRNA (Viral System, Lentivirus) (MTS-1122-YF110)

Cargo Type

CRISPR KO

Specific Cargo

IL-10 gRNA

Target Common Name

IL10

Target Alternative Names

CSIF; TGIF; GVHDS; IL-10; IL10A

Target Full Name

Interleukin 10

Introduction

The protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. Mutations in this gene are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.

UniprotID

GeneID

Cargo Delivery System Type

Viral System

Cargo Delivery Approach

Lentivirus

Applications

Resistant to reprogramming by tumor-secreted signals

References

Moyes, Kara W et al. "Genetically Engineered Macrophages: A Potential Platform for Cancer Immunotherapy." Human gene therapy vol. 28,2 (2017): 200-215. doi:10.1089/hum.2016.060

Mycoplasma Testing

Negative

Sterility Testing

Negative

Shipping

Dry ice

Storage

Frozen cells should be stored in a liquid nitrogen tank (-150°C~-190°C) for long term.

Handling Notes

Frozen cells should be thawed immediately upon receipt and grown according to handling procedure to ensure cell viability and proper assay performance.
Note: Do not freeze the cells upon receipt as it may result in irreversible damage to the cell line.
Disclaimer: We cannot guarantee cell viability if the cells are not thawed immediately upon receipt and grown according to handling procedure.

Restriction

Research use only