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MacroCargo™ Human PBMC-derived Monocytes with CYP2B6 (Viral System, Adenovirus) (MTS-1222-YF490)

Datasheet

Cell Properties

Product Overview

As a therapeutic tool, macrophage cell has a great capacity for delivering cargos because of their intrinsic characteristics. This product is engineered Human PBMC-derived Monocytes carried with CYP2B6 by Viral System-Adenovirus. MacroCargo™ products aim to improve the macrophage function and delivery of specific cargos. We also provide custom macrophage delivery systems based on your specific requirements.

Cell Name

PBMC-derived Monocytes

Cell Type

Primary Cell

Cell Species

Human

Cell Background

Monocytes express various receptors, which monitor and sense environmental changes. Monocytes are highly plastic and heterogeneous, and change their functional phenotype in response to environmental stimulation. Evidence from murine and human studies has suggested that monocytosis can be an indicator of various inflammatory diseases. Monocytes can differentiate into inflammatory or anti-inflammatory subsets. Upon tissue damage or infection, monocytes are rapidly recruited to the tissue, where they can differentiate into tissue macrophages or dendritic cells.

Cargo Properties

Cargo Type

Protein

Specific Cargo

CYP2B6

Cargo Common Name

CYP2B6

Cargo Alternative Names

CPB6; EFVM; IIB1; P450; CYP2B; CYP2B7; CYP2B7P; CYPIIB6

Cargo Full Name

Cytochrome P450 family 2 subfamily B member 6

Introduction

This gene, CYP2B6, encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by phenobarbital. The enzyme is known to metabolize some xenobiotics, such as the anti-cancer drugs cyclophosphamide and ifosphamide. Transcript variants for this gene have been described; however, it has not been resolved whether these transcripts are in fact produced by this gene or by a closely related pseudogene, CYP2B7. Both the gene and the pseudogene are located in the middle of a CYP2A pseudogene found in a large cluster of cytochrome P450 genes from the CYP2A, CYP2B and CYP2F subfamilies on chromosome 19q.

UniprotID

P20813

GeneID

1555

Cargo Delivery System Type

Viral System

Cargo Delivery Approach

Adenovirus

Product Properties

Applications

Enhance chemotherapeutic drugs delivery to hypoxic tumor tissue

References

Griffiths, L et al. "The macrophage - a novel system to deliver gene therapy to pathological hypoxia." Gene therapy vol. 7,3 (2000): 255-62. doi:10.1038/sj.gt.3301058

Mycoplasma Testing

Negative

Sterility Testing

Negative

Shipping

Dry ice

Storage

Frozen cells should be stored in a liquid nitrogen tank (-150°C~-190°C) for long term.

Handling Notes

Frozen cells should be thawed immediately upon receipt and grown according to handling procedure to ensure cell viability and proper assay performance.
Note: Do not freeze the cells upon receipt as it may result in irreversible damage to the cell line.
Disclaimer: We cannot guarantee cell viability if the cells are not thawed immediately upon receipt and grown according to handling procedure.

Restriction

Research use only