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MacroCargo™ Human PBMC-derived Macrophages with SIGLEC9 gRNA (Viral System, Lentivirus) (MTS-1122-YF291)

Datasheet

Cell Properties

Product Overview

As a therapeutic tool, macrophage cell has a great capacity for delivering cargos because of their intrinsic characteristics. This product is engineered Human PBMC-derived Macrophages carried with SIGLEC9 gRNA by Viral System-Lentivirus. MacroCargo™ products aim to improve the macrophage function and delivery of specific cargos. We also provide custom macrophage delivery systems based on your specific requirements.

Cell Name

PBMC-derived Macrophages

Cell Type

Primary Cell

Cell Species

Human

Cell Background

Macrophages are specialised cells involved in the detection, phagocytosis and destruction of bacteria and other harmful organisms. In addition, they can also present antigens to T cells and initiate inflammation by releasing molecules (known as cytokines) that activate other cells. Furthermore, macrophages are able to cross the biological barriers, penetrate into tumors, and accumulate particularly in hypoxic and poorly vascularized portions of a tumor.

Cargo Properties

Cargo Type

CRISPR KO

Specific Cargo

SIGLEC9 gRNA

Target Common Name

SIGLEC9

Target Alternative Names

CD329; CDw329; FOAP-9; siglec-9; OBBP-LIKE

Target Full Name

Sialic acid binding Ig like lectin 9

Introduction

Predicted to enable monosaccharide binding activity and sialic acid binding activity. Predicted to be involved in cell adhesion. Predicted to act upstream of or within negative regulation of inflammatory response and negative regulation of phagocytosis, engulfment. Predicted to be located in external side of plasma membrane. Predicted to be active in plasma membrane.

UniprotID

Q9Y336

GeneID

27180

Cargo Delivery System Type

Viral System

Cargo Delivery Approach

Lentivirus

Product Properties

Applications

Promote the macrophage capability to limit tumor growth

References

Mantovani, Alberto et al. "Macrophages as tools and targets in cancer therapy." Nature reviews. Drug discovery vol. 21,11 (2022): 799-820. doi:10.1038/s41573-022-00520-5

Mycoplasma Testing

Negative

Sterility Testing

Negative

Shipping

Dry ice

Storage

Frozen cells should be stored in a liquid nitrogen tank (-150°C~-190°C) for long term.

Handling Notes

Frozen cells should be thawed immediately upon receipt and grown according to handling procedure to ensure cell viability and proper assay performance.
Note: Do not freeze the cells upon receipt as it may result in irreversible damage to the cell line.
Disclaimer: We cannot guarantee cell viability if the cells are not thawed immediately upon receipt and grown according to handling procedure.

Restriction

Research use only